Hi moayyad,
Please refer below sample.
HTML
<asp:GridView ID="gvCustomers" runat="server" AutoGenerateColumns="false" OnRowDataBound="gvCustomers_OnRowDataBound">
    <Columns>
        <asp:BoundField DataField="Current_Date" HeaderText="Current_Date" />
        <asp:BoundField DataField="End_Date" HeaderText="End_Date" />
    </Columns>
</asp:GridView>
Namespaces
C#
using System.Data;
VB.Net
Imports System.Data
Code
C#
protected void Page_Load(object sender, EventArgs e)
{
    if (!this.IsPostBack)
    {
        DataTable dt = new DataTable();
        dt.Columns.AddRange(new DataColumn[] { new DataColumn("Current_Date", typeof(DateTime)), new DataColumn("End_Date", typeof(DateTime)), new DataColumn("totaldiff", typeof(string)) });
        dt.Rows.Add(DateTime.Now, "11/01/2018");
        dt.Rows.Add(DateTime.Now, "11/02/2018");
        dt.Rows.Add(DateTime.Now, "11/03/2018");
        this.gvCustomers.DataSource = dt;
        this.gvCustomers.DataBind();
    }
}
protected void gvCustomers_OnRowDataBound(object sender, GridViewRowEventArgs e)
{
    if (e.Row.RowType == DataControlRowType.Header)
    {
        TableCell cell = new TableCell();
        cell.Text = "<b>Difference</b>";
        e.Row.Cells.AddAt(gvCustomers.Columns.Count, cell);
    }
    if (e.Row.RowType == DataControlRowType.DataRow)
    {
        DateTime currentdate = Convert.ToDateTime(e.Row.Cells[0].Text);
        int currenthour = currentdate.Hour;
        int currentsecond = currentdate.Second;
        int currentminute = currentdate.Minute;
        DateTime enddate = Convert.ToDateTime(e.Row.Cells[1].Text);
        int day1 = currentdate.Day;
        int day2 = enddate.Day;
        int totalday = day1 - day2;
        int endhour = enddate.Hour;
        int endminute = enddate.Minute;
        int endsecond = enddate.Second;
        int diffhour = currenthour - endhour;
        int diffminute = currentminute - endminute;
        int diffsecond = currentsecond - endsecond;
        string totaldiff = totalday + ":" + diffhour + ":" + diffminute + ":" + diffsecond;
        TableCell cell = new TableCell();
        cell.Text = totaldiff;
        e.Row.Cells.AddAt(gvCustomers.Columns.Count, cell);
    }
}
VB.Net
Protected Sub Page_Load(ByVal sender As Object, ByVal e As EventArgs) Handles Me.Load
    If Not Me.IsPostBack Then
        Dim dt As DataTable = New DataTable()
        dt.Columns.AddRange(New DataColumn() {New DataColumn("Current_Date", GetType(DateTime)), New DataColumn("End_Date", GetType(DateTime)), New DataColumn("totaldiff", GetType(String))})
        dt.Rows.Add(DateTime.Now, "11/01/2018")
        dt.Rows.Add(DateTime.Now, "11/02/2018")
        dt.Rows.Add(DateTime.Now, "11/03/2018")
        Me.gvCustomers.DataSource = dt
        Me.gvCustomers.DataBind()
    End If
End Sub
Protected Sub gvCustomers_OnRowDataBound(ByVal sender As Object, ByVal e As GridViewRowEventArgs) Handles Me.Load
    If e.Row.RowType = DataControlRowType.Header Then
        Dim cell As TableCell = New TableCell()
        cell.Text = "<b>Difference</b>"
        e.Row.Cells.AddAt(gvCustomers.Columns.Count, cell)
    End If
    If e.Row.RowType = DataControlRowType.DataRow Then
        Dim currentdate As DateTime = Convert.ToDateTime(e.Row.Cells(0).Text)
        Dim currenthour As Integer = currentdate.Hour
        Dim currentsecond As Integer = currentdate.Second
        Dim currentminute As Integer = currentdate.Minute
        Dim enddate As DateTime = Convert.ToDateTime(e.Row.Cells(1).Text)
        Dim day1 As Integer = currentdate.Day
        Dim day2 As Integer = enddate.Day
        Dim totalday As Integer = day1 - day2
        Dim endhour As Integer = enddate.Hour
        Dim endminute As Integer = enddate.Minute
        Dim endsecond As Integer = enddate.Second
        Dim diffhour As Integer = currenthour - endhour
        Dim diffminute As Integer = currentminute - endminute
        Dim diffsecond As Integer = currentsecond - endsecond
        Dim totaldiff As String = totalday & ":" & diffhour & ":" & diffminute & ":" & diffsecond
        Dim cell As TableCell = New TableCell()
        cell.Text = totaldiff
        e.Row.Cells.AddAt(gvCustomers.Columns.Count, cell)
    End If
End Sub
Screenshot
