Hi vishalvp,

Using the below article i have created the example.

Check this example. Now please take its reference and correct your code.

Upload and Play (Live Stream) MP4 Video files from Database using HTML5 Video Player in ASP.Net using C# and VB.Net

HTML

Default

<asp:FileUpload ID="FileUpload1" runat="server" />
<asp:Button ID="btnUpload" runat="server" Text="Upload" OnClick="OnUpload" />
<hr />
<asp:Repeater ID="rptVideos" runat="server">
    <HeaderTemplate>
        <table>
    </HeaderTemplate>
    <ItemTemplate>
        <tr>
            <td><asp:Label ID="lblId" runat="server" Width="100%" Text='<%#Eval("Id")%>'></asp:Label></td>
            <td><asp:Label ID="lblName" runat="server" Text='<%# Eval("Name") %>'> </asp:Label></td>
            <td>
                <asp:LinkButton ID="lnkbutton" runat="server" CommandArgument='<%#Eval("Id")%>'
                    OnClick="OnPlay" Text="Play Video" Style="display: block;"></asp:LinkButton>
            </td>
        </tr>
    </ItemTemplate>
    <FooterTemplate>
        </table>
    </FooterTemplate>
</asp:Repeater>

Player

 <video id="VideoPlayer" runat="server" controls="true" width="300" height="300" loop="true" autoplay />

Namespaces

C#

using System.Configuration;
using System.Data;
using System.Data.SqlClient;
using System.IO;
using System.Text;

VB.Net

Imports System.Configuration
Imports System.Data
Imports System.Data.SqlClient
Imports System.IO
Imports System.Text

Code

Default

C#

protected void Page_Load(object sender, EventArgs e)
{
    if (!this.IsPostBack)
    {
        string strConnString = ConfigurationManager.ConnectionStrings["constr"].ConnectionString;
        using (SqlConnection con = new SqlConnection(strConnString))
        {
            using (SqlCommand cmd = new SqlCommand())
            {
                cmd.CommandText = "SELECT id, Name, ContentType, Data FROM tblFiles WHERE ContentType = 'video/mp4'";
                cmd.Connection = con;
                SqlDataAdapter da = new SqlDataAdapter(cmd);
                DataTable dt = new DataTable();
                da.Fill(dt);
                rptVideos.DataSource = dt;
                rptVideos.DataBind();
            }
        }
    }
}

protected void OnUpload(object sender, EventArgs e)
{
    using (BinaryReader br = new BinaryReader(FileUpload1.PostedFile.InputStream))
    {
        byte[] bytes = br.ReadBytes((int)FileUpload1.PostedFile.InputStream.Length);
        string strConnString = ConfigurationManager.ConnectionStrings["constr"].ConnectionString;
        using (SqlConnection con = new SqlConnection(strConnString))
        {
            using (SqlCommand cmd = new SqlCommand())
            {
                cmd.CommandText = "INSERT INTO tblFiles(Name, ContentType, Data) VALUES (@Name, @ContentType, @Data)";
                cmd.Parameters.AddWithValue("@Name", Path.GetFileName(FileUpload1.PostedFile.FileName));
                cmd.Parameters.AddWithValue("@ContentType", "video/mp4");
                cmd.Parameters.AddWithValue("@Data", bytes);
                cmd.Connection = con;
                con.Open();
                cmd.ExecuteNonQuery();
                con.Close();
            }
        }
    }

    Response.Redirect(Request.Url.AbsoluteUri);
}

protected void OnPlay(object sender, EventArgs e)
{
    string url = string.Format("Player.aspx?id={0}", (sender as LinkButton).CommandArgument);
    StringBuilder sb = new StringBuilder();
    sb.Append("<script type = 'text/javascript'>");
    sb.Append("window.open('");
    sb.Append(url);
    sb.Append("');</script>");
    ClientScript.RegisterStartupScript(this.GetType(), "script", sb.ToString());
}

VB.Net

Protected Sub Page_Load(ByVal sender As Object, ByVal e As EventArgs) Handles Me.Load
    If Not Me.IsPostBack Then
        Dim strConnString As String = ConfigurationManager.ConnectionStrings("constr").ConnectionString
        Using con As SqlConnection = New SqlConnection(strConnString)
            Using cmd As SqlCommand = New SqlCommand()
                cmd.CommandText = "SELECT id, Name, ContentType, Data FROM tblFiles WHERE ContentType = 'video/mp4'"
                cmd.Connection = con
                Dim da As SqlDataAdapter = New SqlDataAdapter(cmd)
                Dim dt As DataTable = New DataTable()
                da.Fill(dt)
                rptVideos.DataSource = dt
                rptVideos.DataBind()
            End Using
        End Using
    End If
End Sub

Protected Sub OnUpload(ByVal sender As Object, ByVal e As EventArgs)
    Using br As BinaryReader = New BinaryReader(FileUpload1.PostedFile.InputStream)
        Dim bytes As Byte() = br.ReadBytes(CInt(FileUpload1.PostedFile.InputStream.Length))
        Dim strConnString As String = ConfigurationManager.ConnectionStrings("constr").ConnectionString
        Using con As SqlConnection = New SqlConnection(strConnString)
            Using cmd As SqlCommand = New SqlCommand()
                cmd.CommandText = "INSERT INTO tblFiles(Name, ContentType, Data) VALUES (@Name, @ContentType, @Data)"
                cmd.Parameters.AddWithValue("@Name", Path.GetFileName(FileUpload1.PostedFile.FileName))
                cmd.Parameters.AddWithValue("@ContentType", "video/mp4")
                cmd.Parameters.AddWithValue("@Data", bytes)
                cmd.Connection = con
                con.Open()
                cmd.ExecuteNonQuery()
                con.Close()
            End Using
        End Using
    End Using

    Response.Redirect(Request.Url.AbsoluteUri)
End Sub

Protected Sub OnPlay(ByVal sender As Object, ByVal e As EventArgs)
    Dim url As String = String.Format("Player.aspx?id={0}", (TryCast(sender, LinkButton)).CommandArgument)
    Dim sb As StringBuilder = New StringBuilder()
    sb.Append("<script type = 'text/javascript'>")
    sb.Append("window.open('")
    sb.Append(url)
    sb.Append("');</script>")
    ClientScript.RegisterStartupScript(Me.GetType(), "script", sb.ToString())
End Sub

Player

C#

protected void Page_Load(object sender, EventArgs e)
{
    VideoPlayer.Attributes.Add("src", string.Format("VideoHandler.ashx?Id={0}", Request.QueryString["Id"]));
}

VB.Net

Protected Sub Page_Load(ByVal sender As Object, ByVal e As EventArgs) Handles Me.Load
    VideoPlayer.Attributes.Add("src", String.Format("VideoHandler.ashx?Id={0}", Request.QueryString("Id")))
End Sub

Handler

C#

<%@ WebHandler Language="C#" Class="VideoHandler" %>

using System;
using System.Web;
using System.Configuration;
using System.Data.SqlClient;

public class VideoHandler : IHttpHandler
{
    public void ProcessRequest(HttpContext context)
    {
        try
        {
            int id = int.Parse(context.Request.QueryString["id"]);
            byte[] bytes;
            string contentType;
            string strConnString = ConfigurationManager.ConnectionStrings["constr"].ConnectionString;
            string name;
            using (SqlConnection con = new SqlConnection(strConnString))
            {
                using (SqlCommand cmd = new SqlCommand())
                {
                    cmd.CommandText = "SELECT Name, Data, ContentType FROM tblFiles WHERE Id=@Id";
                    cmd.Parameters.AddWithValue("@Id", id);
                    cmd.Connection = con;
                    con.Open();
                    SqlDataReader sdr = cmd.ExecuteReader();
                    sdr.Read();
                    bytes = (byte[])sdr["Data"];
                    contentType = sdr["ContentType"].ToString();
                    name = sdr["Name"].ToString();
                    con.Close();
                }
            }
            context.Response.Clear();
            context.Response.Buffer = true;
            context.Response.AppendHeader("Content-Disposition", "attachment; filename=" + name);
            context.Response.ContentType = contentType;
            context.Response.BinaryWrite(bytes);
            context.Response.End();
        }
        catch (Exception ex)
        {
            context.Response.Write(ex);
        }
    }

    public bool IsReusable
    {
        get
        {
            return false;
        }
    }
}

VB.Net

<%@ WebHandler Language="VB" Class="VideoHandler" %>

Imports System
Imports System.Web
Imports System.Configuration
Imports System.Data.SqlClient

Public Class VideoHandler : Implements IHttpHandler

    Public Sub ProcessRequest(ByVal context As HttpContext) Implements IHttpHandler.ProcessRequest
        Try
            Dim id As Integer = Integer.Parse(context.Request.QueryString("id"))
            Dim bytes As Byte()
            Dim contentType As String
            Dim strConnString As String = ConfigurationManager.ConnectionStrings("constr").ConnectionString
            Dim name As String
            Using con As SqlConnection = New SqlConnection(strConnString)
                Using cmd As SqlCommand = New SqlCommand()
                    cmd.CommandText = "SELECT Name, Data, ContentType FROM tblFiles WHERE Id=@Id"
                    cmd.Parameters.AddWithValue("@Id", id)
                    cmd.Connection = con
                    con.Open()
                    Dim sdr As SqlDataReader = cmd.ExecuteReader()
                    sdr.Read()
                    bytes = CType(sdr("Data"), Byte())
                    contentType = sdr("ContentType").ToString()
                    name = sdr("Name").ToString()
                    con.Close()
                End Using
            End Using

            context.Response.Clear()
            context.Response.Buffer = True
            context.Response.AppendHeader("Content-Disposition", "attachment; filename=" & name)
            context.Response.ContentType = contentType
            context.Response.BinaryWrite(bytes)
            context.Response.End()
        Catch ex As Exception
            context.Response.Write(ex)
        End Try
    End Sub

    Public ReadOnly Property IsReusable() As Boolean Implements IHttpHandler.IsReusable
        Get
            Return False
        End Get
    End Property
End Class

Screenshot