Please refer below code

HTML

<div>
    <div id="dvQuestion" runat="server">
        <h1>
            <asp:Label ID="lblQuestion" runat="server" />
        </h1>
        <br />
        <asp:RadioButtonList ID="rbtnOptions" runat="server">
        </asp:RadioButtonList>
        <br />
        <br />
        <asp:Button ID="Button1" Text="Next" runat="server" OnClick="Next" />
    </div>
    <div id="dvResult" runat="server" visible="false">
        <h1>
            <asp:Label ID="lblResult" runat="server" />
        </h1>
        <br />
        <asp:Button ID="Button2" Text="Start Again" runat="server" OnClick="Start" />
    </div>
</div>

C#

private DataTable DataTableQuestions
{
    get { return (DataTable)ViewState["Question"]; }
    set { ViewState["Question"] = value; }
}

private int QuestionIndex
{
    get { return (int)ViewState["QuestionIndex"]; }
    set { ViewState["QuestionIndex"] = value; }
}

private int Score
{
    get { return (int)ViewState["Score"]; }
    set { ViewState["Score"] = value; }
}

protected void Page_Load(object sender, EventArgs e)
{
    if (!this.IsPostBack)
    {
        DataTableQuestions = PopulateQuestions();
        QuestionIndex = 0;
        Score = 0;
        GetCurrentQuestion(QuestionIndex, DataTableQuestions);
    }
}

protected void Next(object sender, EventArgs e)
{
    QuestionIndex++;
    GetCurrentQuestion(QuestionIndex, DataTableQuestions, int.Parse(rbtnOptions.SelectedItem.Value));
}

protected void Start(object sender, EventArgs e)
{
    Response.Redirect(Request.Url.AbsoluteUri);
}

private DataTable PopulateQuestions()
{
    DataTable dt = new DataTable();
    string constr = ConfigurationManager.ConnectionStrings["constr"].ConnectionString;
    using (SqlConnection _cn = new SqlConnection(constr))
    {
        using (SqlCommand _cmd = new SqlCommand("SELECT * FROM QuestionTable", _cn))
        {
            using (SqlDataAdapter da = new SqlDataAdapter(_cmd))
            {
                _cn.Open();
                da.Fill(dt);
                _cn.Close();
            }
        }
    }
    return dt;
}

private void GetCurrentQuestion(int index, DataTable dtQuestions, int? selectedOption = null)
{
    if (selectedOption != null)
    {
        string option = string.Empty;
        DataRow row = dtQuestions.Rows[index - 1];
        if (selectedOption == 0)
        {
            option = "OptionOne";
        }
        else if (selectedOption == 1)
        {
            option = "OptionTwo";
        }
        else if (selectedOption == 2)
        {
            option = "OptionThree";
        }
        else if (selectedOption == 3)
        {
            option = "OptionFour";
        }
        if (option == row["CorrectAnswer"].ToString())
        {
            Score++;
        }
    }
    if (index < dtQuestions.Rows.Count)
    {
        DataRow row = dtQuestions.Rows[index];
        lblQuestion.Text = row["QuestionDescription"].ToString();
        List<ListItem> options = new List<ListItem>();
        ListItem option1 = new ListItem(row["OptionOne"].ToString(), "0");
        ListItem option2 = new ListItem(row["OptionTwo"].ToString(), "1");
        ListItem option3 = new ListItem(row["OptionThree"].ToString(), "2");
        ListItem option4 = new ListItem(row["OptionFour"].ToString(), "3");
        options.AddRange(new ListItem[4] { option1, option2, option3, option4 });
        List<ListItem> randomOptions = RandomizeList(options);
        rbtnOptions.Items.Clear();
        rbtnOptions.Items.AddRange(randomOptions.ToArray());
        rbtnOptions.DataBind();
    }
    else
    {
        dvQuestion.Visible = false;
        dvResult.Visible = true;
        lblResult.Text = string.Format("You Scored {0}/{1}", Score, index);
    }
}

public List<ListItem> RandomizeList(List<ListItem> originalList)
{
    List<ListItem> randomList = new List<ListItem>();
    Random random = new Random();
    ListItem value = default(ListItem);
    while (originalList.Count() > 0)
    {
        var nextIndex = random.Next(0, originalList.Count());
        value = originalList[nextIndex];
        randomList.Add(value);
        originalList.RemoveAt(nextIndex);
    }
    return randomList;
}

Screenshot