Hi Waghmare,

I have created a sample by refering the below article.

Auto Refresh UpdatePanel in ASP.Net

HTML

<asp:ScriptManager ID="ScriptManager1" runat="server">
</asp:ScriptManager>
<asp:UpdatePanel ID="UpdatePanel1" runat="server">
    <ContentTemplate>
        <asp:Timer ID="Timer1" runat="server" OnTick="RefreshGridView" Interval="1000" />
        <asp:GridView ID="GridView1" runat="server" AutoGenerateColumns="false">
            <Columns>
                <asp:BoundField DataField="CustomerId" HeaderText="Id" />
                <asp:BoundField DataField="Name" HeaderText="Name" />
                <asp:BoundField DataField="Country" HeaderText="Country" />
            </Columns>
        </asp:GridView>
    </ContentTemplate>
</asp:UpdatePanel>

C#

protected void Page_Load(object sender, EventArgs e)
{
    if (!this.IsPostBack)
    {
        BindGridView();
    }
}

private void BindGridView()
{
    using (SqlConnection con = new SqlConnection(ConfigurationManager.ConnectionStrings["constr"].ConnectionString))
    {
        using (SqlCommand cmd = new SqlCommand())
        {
            cmd.Connection = con;
            cmd.CommandType = CommandType.Text;
            cmd.CommandText = "SELECT * FROM CustomerTest";
            con.Open();
            GridView1.DataSource = cmd.ExecuteReader();
            GridView1.DataBind();
            con.Close();
        }
    }
}

protected void RefreshGridView(object sender, EventArgs e)
{
    BindGridView();
}

VB.Net

Protected Sub Page_Load(sender As Object, e As EventArgs) Handles Me.Load
    If Not Me.IsPostBack Then
        BindGridView()
    End If
End Sub

Private Sub BindGridView()
    Using con As New SqlConnection(ConfigurationManager.ConnectionStrings("constr").ConnectionString)
        Using cmd As New SqlCommand()
            cmd.Connection = con
            cmd.CommandType = CommandType.Text
            cmd.CommandText = "SELECT * FROM CustomerTest"
            con.Open()
            GridView1.DataSource = cmd.ExecuteReader()
            GridView1.DataBind()
            con.Close()
        End Using
    End Using
End Sub

Protected Sub RefreshGridView(sender As Object, e As EventArgs)
    BindGridView()
End Sub

Screenshot