Refer this HTML code
HTML
<cc1:ToolkitScriptManager runat="server">
</cc1:ToolkitScriptManager>
hh:mm:ss
<asp:TextBox ID="txtTime" runat="server"></asp:TextBox>
<cc1:MaskedEditExtender ID="MaskedEditExtender1" runat="server" MaskType="Time" UserTimeFormat="TwentyFourHour"
Mask="99:99:99" TargetControlID="txtTime">
</cc1:MaskedEditExtender>
Screenshot

Empty TextBox
