When Master Page is used the ASP.Net AJAX MultiSliderExtender is not able to find the HiddenField.
Hence you need to follow the steps
1. Do Page View Source and copy the ClientIDs of Hidden Fields.
2. Now use this ClientID as Target.
<asp:MultiHandleSliderExtender ID="MultiHandleSliderExtender1" runat="server" TargetControlID="txtSlider"
Minimum="1" Maximum="1000" Increment="1" RaiseChangeOnlyOnMouseUp="true" EnableRailClick="false"
Length="1000" OnClientDragEnd="OnClientDragEnd">
<multihandleslidertargets>
<asp:MultiHandleSliderTarget ControlID="ContentPlaceHolder1_hfStart" />
<asp:MultiHandleSliderTarget ControlID="ContentPlaceHolder1_hfEnd" />
</multihandleslidertargets>
</asp:MultiHandleSliderExtender>