Hi alhamd,

Use below article i have created the example.

Upload and Play MP3 Audio files from Database using HTML5 Audio Player in ASP.Net

Refer below code.

HTML

<div class="container py-3">
    <h2 class="text-center text-uppercase">How to upload video in database and play using asp.net</h2>
    <div class="row">
        <div class="form-group">
            <label>Choose Video:</label>
            <div class="input-group">
                <div class="custom-file">
                    <asp:FileUpload ID="FileUpload1" CssClass="custom-file-input" runat="server" AllowMultiple="true" />
                    <label class="custom-file-label"></label>
                </div>
                <div class="input-group-append">
                    <asp:Button ID="btnUpload" CssClass="btn btn-outline-secondary" runat="server" Text="Upload" OnClick="btnUpload_Click" />
                </div>
            </div>
        </div>
    </div>
    <asp:Label ID="lblMessage" runat="server"></asp:Label>
    <asp:GridView ID="GridView1" ShowHeaderWhenEmpty="true" HeaderStyle-CssClass="bg-primary text-white" runat="server" AutoGenerateColumns="false" CssClass="table table-bordered">
        <EmptyDataTemplate>
            <div class="text-center">No Data Found <strong>Upload New Video</strong></div>
        </EmptyDataTemplate>
        <Columns>
            <asp:BoundField HeaderText="ID" DataField="VID" Visible="false" />
            <asp:BoundField HeaderText="Name" DataField="FileName" />
            <asp:TemplateField HeaderText="">
                <ItemTemplate>
                    <video width="130" height="130" controls runat="server" visible='<%# Eval("ContentType").ToString().ToLower()=="video/mp4" ? true : false %>'>
                        <source src='<%# Eval("Id", "File.ashx?Id={0}") %>' type="video/mp4">
                    </video>
                    <audio controls runat="server" visible='<%# Eval("ContentType").ToString().ToLower()=="audio/mpeg3" ? true : false %>'>
                        <source src='<%# Eval("Id", "File.ashx?Id={0}") %>' type='audio/mpeg3' />
                    </audio>
                </ItemTemplate>
            </asp:TemplateField>
            <asp:HyperLinkField DataNavigateUrlFields="Id" Text="Download" DataNavigateUrlFormatString="~/File.ashx?Id={0}"
                HeaderText="Download" />
        </Columns>
    </asp:GridView>
</div>

Namespaces

C#

using System.IO;
using System.Data.SqlClient;
using System.Configuration;

VB.Net

Imports System.IO
Imports System.Data.SqlClient
Imports System.Configuration

Code

C#

protected void Page_Load(object sender, EventArgs e)
{
    if (!IsPostBack)
    {
        this.BindGrid();
    }
}

protected void btnUpload_Click(object sender, EventArgs e)
{
    foreach (HttpPostedFile postedFile in FileUpload1.PostedFiles)
    {
        string filename = Path.GetFileName(postedFile.FileName);
        string contentType = postedFile.ContentType;
        using (Stream fs = postedFile.InputStream)
        {
            using (BinaryReader br = new BinaryReader(fs))
            {
                byte[] bytes = br.ReadBytes((Int32)fs.Length);
                string constr = ConfigurationManager.ConnectionStrings["constr"].ConnectionString;
                using (SqlConnection con = new SqlConnection(constr))
                {
                    string query = "insert into tblFiles values (@Name, @ContentType, @Data)";
                    using (SqlCommand cmd = new SqlCommand(query))
                    {
                        cmd.Connection = con;
                        cmd.Parameters.AddWithValue("@Name", filename);
                        cmd.Parameters.AddWithValue("@ContentType", contentType);
                        cmd.Parameters.AddWithValue("@Data", bytes);
                        con.Open();
                        cmd.ExecuteNonQuery();
                        con.Close();
                    }
                }
            }
        }
    }

    Response.Redirect(Request.Url.AbsoluteUri);
}

private void BindGrid()
{
    string constr = ConfigurationManager.ConnectionStrings["constr"].ConnectionString;
    using (SqlConnection con = new SqlConnection(constr))
    {
        using (SqlCommand cmd = new SqlCommand())
        {
            cmd.CommandText = "SELECT * FROM tblFiles";
            cmd.Connection = con;
            con.Open();
            GridView1.DataSource = cmd.ExecuteReader();
            GridView1.DataBind();
            con.Close();
        }
    }
}

VB.Net

Protected Sub Page_Load(ByVal sender As Object, ByVal e As EventArgs) Handles Me.Load
    If Not IsPostBack Then
        Me.BindGrid()
    End If
End Sub

Protected Sub btnUpload_Click(ByVal sender As Object, ByVal e As EventArgs)
    For Each postedFile As HttpPostedFile In FileUpload1.PostedFiles
        Dim filename As String = Path.GetFileName(postedFile.FileName)
        Dim contentType As String = postedFile.ContentType
        Using fs As Stream = postedFile.InputStream
            Using br As BinaryReader = New BinaryReader(fs)
                Dim bytes As Byte() = br.ReadBytes(CType(fs.Length, Int32))
                Dim constr As String = ConfigurationManager.ConnectionStrings("constr").ConnectionString
                Using con As SqlConnection = New SqlConnection(constr)
                    Dim query As String = "insert into tblFiles values (@Name, @ContentType, @Data)"
                    Using cmd As SqlCommand = New SqlCommand(query)
                        cmd.Connection = con
                        cmd.Parameters.AddWithValue("@Name", filename)
                        cmd.Parameters.AddWithValue("@ContentType", contentType)
                        cmd.Parameters.AddWithValue("@Data", bytes)
                        con.Open()
                        cmd.ExecuteNonQuery()
                        con.Close()
                    End Using
                End Using
            End Using
        End Using
    Next

    Response.Redirect(Request.Url.AbsoluteUri)
End Sub

Private Sub BindGrid()
    Dim constr As String = ConfigurationManager.ConnectionStrings("constr").ConnectionString
    Using con As SqlConnection = New SqlConnection(constr)
        Using cmd As SqlCommand = New SqlCommand()
            cmd.CommandText = "SELECT * FROM tblFiles"
            cmd.Connection = con
            con.Open()
            GridView1.DataSource = cmd.ExecuteReader()
            GridView1.DataBind()
            con.Close()
        End Using
    End Using
End Sub

Handler

C#

<%@ WebHandler Language="C#" Class="File" %>

using System;
using System.Web;
using System.Data.SqlClient;
using System.Configuration;

public class File : IHttpHandler
{
    public void ProcessRequest(HttpContext context)
    {
        int id = int.Parse(context.Request.QueryString["id"]);
        byte[] bytes;
        string contentType;
        string strConnString = ConfigurationManager.ConnectionStrings["constr"].ConnectionString;
        string name;
        using (SqlConnection con = new SqlConnection(strConnString))
        {
            using (SqlCommand cmd = new SqlCommand())
            {
                cmd.CommandText = "SELECT Name, Data, ContentType FROM tblFiles WHERE Id=@Id";
                cmd.Parameters.AddWithValue("@Id", id);
                cmd.Connection = con;
                con.Open();
                SqlDataReader sdr = cmd.ExecuteReader();
                sdr.Read();
                bytes = (byte[])sdr["Data"];
                contentType = sdr["ContentType"].ToString();
                name = sdr["Name"].ToString();
                con.Close();
            }
        }
        context.Response.Clear();
        context.Response.Buffer = true;
        context.Response.AppendHeader("Content-Disposition", "attachment; filename=" + name);
        context.Response.ContentType = contentType;
        context.Response.BinaryWrite(bytes);
        context.Response.End();
    }

    public bool IsReusable
    {
        get
        {
            return false;
        }
    }
}

VB.Net

<%@ WebHandler Language="VB" Class="File" %>

Imports System
Imports System.Web
Imports System.Data.SqlClient
Imports System.Configuration

Public Class File : Implements IHttpHandler
    Public Sub ProcessRequest(ByVal context As HttpContext) Implements IHttpHandler.ProcessRequest
        Dim id As Integer = Integer.Parse(context.Request.QueryString("id"))
        Dim bytes As Byte()
        Dim contentType As String
        Dim strConnString As String = ConfigurationManager.ConnectionStrings("constr").ConnectionString
        Dim name As String
        Using con As New SqlConnection(strConnString)
            Using cmd As New SqlCommand()
                cmd.CommandText = "SELECT Name, Data, ContentType FROM tblFiles WHERE Id=@Id"
                cmd.Parameters.AddWithValue("@Id", id)
                cmd.Connection = con
                con.Open()
                Dim sdr As SqlDataReader = cmd.ExecuteReader()
                sdr.Read()
                bytes = DirectCast(sdr("Data"), Byte())
                contentType = sdr("ContentType").ToString()
                name = sdr("Name").ToString()
                con.Close()
            End Using
        End Using
        context.Response.Clear()
        context.Response.Buffer = True
        context.Response.AppendHeader("Content-Disposition", "attachment; filename=" + name)
        context.Response.ContentType = contentType
        context.Response.BinaryWrite(bytes)
        context.Response.[End]()
    End Sub

    Public ReadOnly Property IsReusable() As Boolean Implements IHttpHandler.IsReusable
        Get
            Return False
        End Get
    End Property
End Class

Screenshot