Hi pratikshir,

You need to set Default documents for your website.

Refer below article.

Set default page for your website in IIS using Web.Config file in ASP.Net

You need to add defaultDocument within the system.webServer section.

<?xml version="1.0"?>
<!--
  For more information on how to configure your ASP.NET application, please visit
  http://go.microsoft.com/fwlink/?LinkId=169433
  -->
<configuration>
  <system.webServer>
    <validation validateIntegratedModeConfiguration="false" />
    <modules runAllManagedModulesForAllRequests="true" />
    <defaultDocument enabled="true">
      <files>
        <clear/>
        <add value="Home.aspx"/>
      </files>
    </defaultDocument>
  </system.webServer>
  <system.web>
    <customErrors mode="Off" />
    <compilation debug="true" defaultLanguage="c#" />
  </system.web>
  <connectionStrings>
    <add name="constr" connectionString="Data Source=(local);Initial Catalog=MetaTagsDB;User id = sa;password=123" />
  </connectionStrings>
  <!--Data Source=.;Initial Catalog=Kaaryashala;Integrated Security=True-->
</configuration>