Here I have created sample that will help you out.

I referred below article

Bind CheckBoxList from Database in ASP.Net

HTML

<div>
    <asp:CheckBoxList ID="chkFruits" runat="server">
    </asp:CheckBoxList>
</div>

C#

protected void Page_Load(object sender, EventArgs e)
{
    if (!this.IsPostBack)
    {
        string checkBoxValues = "Mango,Orange,Graps,Apple,Banana";
        foreach (string value in checkBoxValues.Split(','))
        {
            ListItem item = new ListItem();
            item.Text = value;
            item.Value = value;
            chkFruits.Items.Add(item);
        }
    }
}

VB

Protected Sub Page_Load(sender As Object, e As EventArgs)
	If Not Me.IsPostBack Then
		Dim checkBoxValues As String = "Mango,Orange,Graps,Apple,Banana"
		For Each value As String In checkBoxValues.Split(","C)
			Dim item As New ListItem()
			item.Text = value
			item.Value = value
			chkFruits.Items.Add(item)
		Next
	End If
End Sub

Screenshot