New version of AjaxControlToolkit does not support ScriptManager and hence you need to use ToolkitScriptManager which is part of AjaxControlToolkit controls.
1. Add reference of AjaxControlToolkit and then register it as shown below.
<%@ Register Assembly="AjaxControlToolkit" Namespace="AjaxControlToolkit" TagPrefix="asp" %>
2. Now remove ScriptManager and add ToolkitScriptManager.
<asp:ToolkitScriptManager ID="ToolkitScriptManager1" runat="server"> </asp:ToolkitScriptManager>