ASP.Net RegularExpressionValidator ValidationExpression to restrict specified charterers

Hi

I want to create a ValidationExpression code for allow charterers and number except following special charterers;

# pound

% percent

& ampersand

{ left curly bracket

} right curly bracket

\ back slash

< left angle bracket

> right angle bracket

* asterisk

? question mark

/ forward slash

  blank spaces

$ dollar sign

! exclamation point

' single quotes

" double quotes

: colon

@ at sign

+ plus sign

` backtick

| pipe

= equal sign

<asp:RegularExpressionValidator ID="RegularExpressionValidator3" runat="server"
    ErrorMessage="Special characters no allowed."
    Display="Dynamic" ForeColor="red" SetFocusOnError="True"
    ControlToValidate="TxtImageTital"
    ValidationExpression="^[a-zA-Z0-9_,.\s-]+$">*</asp:RegularExpressionValidator>

Thanking You

samsmuthu