DateTime Difference

Ok Gokuldas you can try this

 DateTime ObjLocDate1 = new DateTime();
        DateTime ObjLocDate2 = new DateTime();
        string StrLocTime = string.Empty;
        ObjLocDate1 = Convert.ToDateTime(txtDate1.Text);
        ObjLocDate2 = Convert.ToDateTime(txtDate2.Text);
        StrLocTime = string.Format("{0}:{1}:{2}", ObjLocDate1.Subtract(ObjLocDate2).Hours.ToString(), ObjLocDate1.Subtract(ObjLocDate2).Minutes.ToString(), ObjLocDate1.Subtract(ObjLocDate2).Seconds.ToString());
        lblDate.Text = StrLocTime;

In this example i have simply passed the hard code values to the  textbox.

But in you case you will have to fetch the time from you table