Hi Hariom,
Use RegularExpression Validator.
Check this example. Now please take its reference and correct your code.
HTML
<asp:TextBox ID="TextBox1" runat="server" /><br />
<asp:RegularExpressionValidator ID="RegularExpressionValidator1" runat="server" ControlToValidate="TextBox1"
ForeColor="Red" ValidationExpression="[ABCD]*$" ErrorMessage="*Valid characters: A,B,C,D." /><br />
<asp:Button ID="Button1" Text="Validate" runat="server" />
Screenshot
![](https://i.imgur.com/ZDlv2Tt.gif)