Refer below links.

how to update image stored on disk and path in database in asp.net gridview

Using ASP.Net AJAX AsyncFileUpload control inside GridView TemplateField

Getting issue Postedfile is not member of Fileupload..pls guide

 Protected Sub GridView1_RowUpdating(sender As Object, e As GridViewUpdateEventArgs)
        Dim file As HttpPostedFile = (CType(GridView1.Rows(e.RowIndex).FindControl("FileUpload1"), fileupload)).PostedFile
        Dim fileName As String = Path.GetFileName(file.FileName)
        Dim oldFileName As String = GridView1.Rows(e.RowIndex).Cells(1).Text
        Dim strConnString As String = System.Configuration.ConfigurationManager.ConnectionStrings("conString").ConnectionString
        Dim con As SqlConnection = New SqlConnection(strConnString)
        Dim strQuery As String = "update tblFiles set FileName=@FileName, FilePath=@FilePath where ID=@ID"
        Dim cmd As SqlCommand = New SqlCommand(strQuery)
        cmd.Parameters.AddWithValue("@ID", GridView1.Rows(e.RowIndex).Cells(0).Text)
        cmd.Parameters.AddWithValue("@FileName", Path.GetFileName(file.FileName))
        cmd.Parameters.AddWithValue("@FilePath", "images/" & fileName)
        cmd.CommandType = CommandType.Text
        cmd.Connection = con
        con.Open()
        cmd.ExecuteNonQuery()
        con.Close()
        file.Delete(Server.MapPath("~/images/") & oldFileName)
        file.SaveAs(Server.MapPath("~/images/") & fileName)
        GridView1.EditIndex = -1
        Response.Redirect(Request.Url.AbsoluteUri)
    End Sub

I am checking it, Will get back to you soon.

Hi manoj07,

Please refer below sample.

HTML

<asp:GridView ID="GridView1" runat="server" AutoGenerateColumns="false" OnRowEditing="OnRowEditing"
    OnRowCancelingEdit="OnRowCancelingEdit" OnRowUpdating="OnRowUpdating">
    <Columns>
        <asp:TemplateField>
            <ItemTemplate>
                <asp:CheckBox ID="ApproveBox" runat="server" />
            </ItemTemplate>
        </asp:TemplateField>
        <asp:BoundField DataField="ID" HeaderText="ID" ReadOnly="true" />
        <asp:BoundField DataField="Name" HeaderText="List of Documents to be Uploaded" ReadOnly="true" />
        <asp:TemplateField>
            <ItemTemplate>
                <asp:FileUpload ID="UploadImages1" runat="server" />
            </ItemTemplate>
        </asp:TemplateField>
        <asp:CommandField ShowEditButton="True" />
    </Columns>
</asp:GridView>

Namespaces

C#

using System.Data;
using System.Data.SqlClient;
using System.IO;

VB.Net

Imports System.IO
Imports System.Data.SqlClient
Imports System.Data

Code

C#

protected void Page_Load(object sender, EventArgs e)
{
    DataTable dt = new DataTable();
    String strConnString = System.Configuration.ConfigurationManager.ConnectionStrings["constr"].ConnectionString;
    string strQuery = "select * from tblFilesPath";
    SqlCommand cmd = new SqlCommand(strQuery);
    SqlConnection con = new SqlConnection(strConnString);
    SqlDataAdapter sda = new SqlDataAdapter();
    cmd.CommandType = CommandType.Text;
    cmd.Connection = con;
    sda.SelectCommand = cmd;
    sda.Fill(dt);
    GridView1.DataSource = dt;
    GridView1.DataBind();

}

protected void OnRowEditing(object sender, GridViewEditEventArgs e)
{
    GridView1.EditIndex = e.NewEditIndex;
    GridView1.DataBind();
}

protected void OnRowCancelingEdit(object sender, GridViewCancelEditEventArgs e)
{
    GridView1.EditIndex = -1;
    GridView1.DataBind();
}

protected void OnRowUpdating(object sender, GridViewUpdateEventArgs e)
{
    HttpPostedFile file = ((FileUpload)GridView1.Rows[e.RowIndex].FindControl("UploadImages1")).PostedFile;
    string fileName = Path.GetFileName(file.FileName);
    string oldFileName = GridView1.Rows[e.RowIndex].Cells[2].Text;
    string strConnString = System.Configuration.ConfigurationManager.ConnectionStrings["constr"].ConnectionString;
    SqlConnection con = new SqlConnection(strConnString);
    string strQuery = "update tblFilesPath set Name=@FileName, Path=@FilePath where ID=@ID";
    SqlCommand cmd = new SqlCommand(strQuery);
    cmd.Parameters.AddWithValue("@ID", GridView1.Rows[e.RowIndex].Cells[1].Text);
    cmd.Parameters.AddWithValue("@FileName", Path.GetFileName(file.FileName));
    cmd.Parameters.AddWithValue("@FilePath", "images/" + fileName);
    cmd.CommandType = CommandType.Text;
    cmd.Connection = con;
    con.Open();
    cmd.ExecuteNonQuery();
    con.Close();
    File.Delete(Server.MapPath("~/images/") + oldFileName);
    file.SaveAs(Server.MapPath("~/images/") + fileName);
    GridView1.EditIndex = -1;
    Response.Redirect(Request.Url.AbsoluteUri);
}

VB.Net

Protected Sub Page_Load(ByVal sender As Object, ByVal e As EventArgs) Handles Me.Load
    Dim dt As DataTable = New DataTable()
    Dim strConnString As String = System.Configuration.ConfigurationManager.ConnectionStrings("constr").ConnectionString
    Dim strQuery As String = "select * from tblFilesPath"
    Dim cmd As SqlCommand = New SqlCommand(strQuery)
    Dim con As SqlConnection = New SqlConnection(strConnString)
    Dim sda As SqlDataAdapter = New SqlDataAdapter()
    cmd.CommandType = CommandType.Text
    cmd.Connection = con
    sda.SelectCommand = cmd
    sda.Fill(dt)
    GridView1.DataSource = dt
    GridView1.DataBind()
End Sub

Protected Sub OnRowEditing(ByVal sender As Object, ByVal e As GridViewEditEventArgs)
    GridView1.EditIndex = e.NewEditIndex
    GridView1.DataBind()
End Sub

Protected Sub OnRowCancelingEdit(ByVal sender As Object, ByVal e As GridViewCancelEditEventArgs)
    GridView1.EditIndex = -1
    GridView1.DataBind()
End Sub

Protected Sub OnRowUpdating(ByVal sender As Object, ByVal e As GridViewUpdateEventArgs)
    Dim file As HttpPostedFile = (CType(GridView1.Rows(e.RowIndex).FindControl("UploadImages1"), FileUpload)).PostedFile
    Dim fileName As String = Path.GetFileName(file.FileName)
    Dim oldFileName As String = GridView1.Rows(e.RowIndex).Cells(2).Text
    Dim strConnString As String = System.Configuration.ConfigurationManager.ConnectionStrings("constr").ConnectionString
    Dim con As SqlConnection = New SqlConnection(strConnString)
    Dim strQuery As String = "update tblFilesPath set Name=@FileName, Path=@FilePath where ID=@ID"
    Dim cmd As SqlCommand = New SqlCommand(strQuery)
    cmd.Parameters.AddWithValue("@ID", GridView1.Rows(e.RowIndex).Cells(1).Text)
    cmd.Parameters.AddWithValue("@FileName", Path.GetFileName(file.FileName))
    cmd.Parameters.AddWithValue("@FilePath", "images/" & fileName)
    cmd.CommandType = CommandType.Text
    cmd.Connection = con
    con.Open()
    cmd.ExecuteNonQuery()
    con.Close()
    System.IO.File.Delete(Server.MapPath("~/images/") & oldFileName)
    file.SaveAs(Server.MapPath("~/images/") & fileName)
    GridView1.EditIndex = -1
    Response.Redirect(Request.Url.AbsoluteUri)
End Sub

Screenshot

Hello Pandeyism,

Thanx for your Promt reply,

But still i am getting error on folowing line 'Posted File is not member of file upload' ...please help

Dim file As HttpPostedFile = (CType(GridView2.Rows(e.RowIndex).FindControl("UploadImages1"), fileupload)).PostedFile