Hi,
I got the solution by my self.
to check if radio butto list is not selcted.
I use below code.
if (radiobuttionlist1.SelectedValue == "")
{
ScriptManager.RegisterStartupScript(this.Page, this.GetType(), "tmp", "<script type='text/javascript'>alert(\"Please select your option for radio button list.\");</script>", false);
return;
}